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ProductsBack/PRMT6 Mouse Monoclonal Antibody [Clone ID: 4G2]
Western blot analysis using PRMT6 mouse mAb against A431 (1), Hela (2), A549 (3) and HEK293 (4) cell lysate.
Western blot analysis using PRMT6 mouse mAb against A431 (1), Hela (2), A549 (3) and HEK293 (4) cell lysate.
Western blot analysis using PRMT6 mouse mAb against A431 (1), Hela (2), A549 (3) and HEK293 (4) cell lysate.

PRMT6 Mouse Monoclonal Antibody [Clone ID: 4G2]

AM06482SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey
TargetPRMT6
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PRMT6 Mouse Monoclonal Antibody [Clone ID: 4G2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4G2
  • Gene ID55170
  • Target name
    PRMT6
  • Target description
    protein arginine methyltransferase 6
  • Target synonyms
    HRMT1L6, protein arginine N-methyltransferase 6, HMT1 hnRNP methyltransferase-like 6, heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, histone-arginine N-methyltransferase PRMT6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96LA8
  • Protein Name
    Protein arginine N-methyltransferase 6
  • Scientific Description
    PRMT6 mouse monoclonal antibody, clone 4G2, Ascites
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRMT6 using anti-PRMT6 antibody (A02924-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT6 antigen affinity purified polyclonal antibody (Catalog # A02924-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT6 at approximately 40 kDa. The expected band size for PRMT6 is at 40 kDa.
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