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Gel: 10%SDS-PAGE, Lysate: 50 ug, Lane: Human liver cancer tissue, Primary antibody: CSB-PA036451(PRMT7 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 10%SDS-PAGE, Lysate: 50 ug, Lane: Human liver cancer tissue, Primary antibody: CSB-PA036451(PRMT7 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 10%SDS-PAGE, Lysate: 50 ug, Lane: Human liver cancer tissue, Primary antibody: CSB-PA036451(PRMT7 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes

PRMT7 Antibody

CSB-PA036451
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPRMT7
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRMT7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54496
  • Target name
    PRMT7
  • Target description
    protein arginine methyltransferase 7
  • Target synonyms
    [Myelin basic protein]-arginine N-methyltransferase PRMT7; histone-arginine N-methyltransferase PRMT7; protein arginine N-methyltransferase 7; SBIDDS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVM4
  • Protein Name
    Protein arginine N-methyltransferase 7
  • Scientific Description
    Protein arginine methyltransferase 7 is a protein that in humans is encoded by the PRMT7 gene. Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing. May be involved in etoposide-induced cytotoxicity, a chemotherapeutic agent frequently used for testicular cancer and small-cell lung cancer that can cause cytotoxicity in the treatment of other cancers. Down-regulation confers increased sensitivity to the Top1 inhibitor camptothecin (CPT).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRMT7 using anti-PRMT7 antibody (A05485-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT7 antigen affinity purified polyclonal antibody (Catalog # A05485-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT7 at approximately 78 kDa. The expected band size for PRMT7 is at 78 kDa.
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