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Western Blot analysis of K562 cells using CD201 Polyclonal Antibody
Western Blot analysis of K562 cells using CD201 Polyclonal Antibody
Western Blot analysis of K562 cells using CD201 Polyclonal Antibody

PROCR Antibody

CSB-PA005324
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPROCR
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Overview

  • Supplier
    Cusabio
  • Product Name
    PROCR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10544
  • Target name
    PROCR
  • Target description
    protein C receptor
  • Target synonyms
    activated protein C receptor; APC receptor; CCCA; CCD41; CD201 antigen; cell cycle, centrosome-associated protein; centrocyclin; endothelial protein C receptor; EPCR; protein C receptor, endothelial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNN8
  • Protein Name
    Endothelial protein C receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EPCR/CD201 using anti-EPCR/CD201 antibody (PB9880). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCR/CD201 antigen affinity purified polyclonal antibody (Catalog # PB9880) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCR/CD201 at approximately 46 kDa, 52 kDa. The expected band size for EPCR/CD201 is at 27 kDa.
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