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ProductsBack/PROCR / EPCR Antibody (Internal)

PROCR / EPCR Antibody (Internal)

LS-C368475
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPROCR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PROCR / EPCR Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10544
  • Target name
    PROCR
  • Target description
    protein C receptor
  • Target synonyms
    activated protein C receptor; APC receptor; CCCA; CCD41; CD201 antigen; cell cycle, centrosome-associated protein; centrocyclin; endothelial protein C receptor; EPCR; protein C receptor, endothelial
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EPCR/CD201 using anti-EPCR/CD201 antibody (PB9880). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCR/CD201 antigen affinity purified polyclonal antibody (Catalog # PB9880) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCR/CD201 at approximately 46 kDa, 52 kDa. The expected band size for EPCR/CD201 is at 27 kDa.
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