![ICC/IF analysis of MCF-7 cells using GTX80698 Progesterone Receptor (phospho Ser294) antibody [608]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC ICC/IF analysis of MCF-7 cells using GTX80698 Progesterone Receptor (phospho Ser294) antibody [608]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX80698/GTX80698_903_ICC-IF_w_23061322_720.webp)
ICC/IF analysis of MCF-7 cells using GTX80698 Progesterone Receptor (phospho Ser294) antibody [608]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
Progesterone Receptor (phospho Ser294) antibody [608]
GTX80698
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPGR
Overview
- SupplierGeneTex
- Product NameProgesterone Receptor (phospho Ser294) antibody [608]
- Delivery Days Customer9
- Application Supplier NoteWB: 10 microg/ml. ICC/IF: 1:20. IHC-P: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID608
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5241
- Target namePGR
- Target descriptionprogesterone receptor
- Target synonymsNR3C3, PR, progesterone receptor, nuclear receptor subfamily 3 group C member 3
- HostMouse
- IsotypeIgG1
- Protein IDP06401
- Protein NameProgesterone receptor
- Scientific DescriptionThis gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![IHC-P analysis of human tonsil tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX30164/GTX30164_1329_IHC-P_w_23060722_507.webp)
![Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294 using Progesterone Receptor (phospho Ser294) antibody [608] (GTX30165). The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).](https://www.genetex.com/upload/website/prouct_img/normal/GTX30165/Progesterone-Receptor-phospho-Ser294-antibody-608__GTX30165-1_w_23060722_991.webp)

![IHC-P analysis of human fibroadenoma (serial sections) tissue using GTX01924 Progesterone Receptor antibody [16 + SAN27]. Note a smaller proportion of weakly staining tumor cell nuclei in A compared to B.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01924/GTX01924_20200811_IHC-P_74_w_23053121_180.webp)
![IHC-P analysis of breast carcinoma tissue using GTX01926 Progesterone Receptor antibody [16]. Note the nuclear staining in a proportion of tumor cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01926/GTX01926_20200811_IHC-P_75_w_23053121_418.webp)
![WB analysis of truncated MBP-PGR recombinant protein (1) andtruncated Trx-PGR(aa730-871) recombinant protein (2) using GTX83165 Progesterone Receptor antibody [8A11H1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83165/GTX83165_20170912_WB_w_23061322_973.webp)