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ProductsBack/Prolactin Receptor antibody [PRLR742]
IHC-P analysis of human testicular carcinoma tissue using GTX34986 Prolactin Receptor antibody [PRLR742].
IHC-P analysis of human testicular carcinoma tissue using GTX34986 Prolactin Receptor antibody [PRLR742].
IHC-P analysis of human testicular carcinoma tissue using GTX34986 Prolactin Receptor antibody [PRLR742].

Prolactin Receptor antibody [PRLR742]

GTX34986
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRLR
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Overview

  • Supplier
    GeneTex
  • Product Name
    Prolactin Receptor antibody [PRLR742]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1-2microg/ml for 30 minutes at RT. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PRLR742
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5618
  • Target name
    PRLR
  • Target description
    prolactin receptor
  • Target synonyms
    HPRL, MFAB, RI-PRLR, hPRLrI, prolactin receptor, hPRL receptor, secreted prolactin binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16471
  • Protein Name
    Prolactin receptor
  • Scientific Description
    This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRLR using anti-PRLR antibody (PB9782). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PB9782) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 65, 95 kDa. The expected band size for PRLR is at 70 kDa.
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