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ProductsBack/Prostatic Acid Phosphatase antibody [PASE-4LJ] (ready-to-use)

Prostatic Acid Phosphatase antibody [PASE-4LJ] (ready-to-use)

GTX73444
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetACP3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Prostatic Acid Phosphatase antibody [PASE/4LJ] (ready-to-use)
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PASE-4LJ
  • Conjugate
    Unconjugated
  • Gene ID55
  • Target name
    ACP3
  • Target description
    acid phosphatase 3
  • Target synonyms
    5'-NT; acid phosphatase, prostate; ACP-3; ACPP; ecto-5'-nucleotidase; prostatic acid phosphatase; prostatic acid phosphotase; protein tyrosine phosphatase ACP3; thiamine monophosphatase; TM-PAP; TMPase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15309
  • Protein Name
    Prostatic acid phosphatase
  • Scientific Description
    This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (Catalog # A02082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa.
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