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Protein C Antibody (YA2461, Rabbit)

HY-P82716
MedChem Express
TargetPROC
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Protein C Antibody (YA2461, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5624
  • Target name
    PROC
  • Target description
    protein C, inactivator of coagulation factors Va and VIIIa
  • Target synonyms
    APC, PC, PROC1, THPH3, THPH4, vitamin K-dependent protein C, Protein C-Nagoya, activated protein C, anticoagulant protein C, autoprothrombin IIA, blood coagulation factor XIV, coagulation factor XIV, prepro-protein C, type I protein C
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Protein C Antibody (YA2461) is a non-conjugated IgG antibody, targeting Protein C, with a predicted molecular weight of 52 kDa (observed band size: 52 kDa). Protein C Antibody (YA2461) can be used for WB experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Protein C using anti-Protein C antibody (A01742). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody (Catalog # A01742) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD.
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