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WB analysis of various sample lysates using GTX66404 Protein S antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66404 Protein S antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66404 Protein S antibody. Dilution : 1:1000 Loading : 25microg per lane

Protein S antibody

GTX66404
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPROS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Protein S antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5627
  • Target name
    PROS1
  • Target description
    protein S
  • Target synonyms
    PROS; protein S (alpha); protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; THPH5; THPH6; vitamin K-dependent plasma protein S; vitamin K-dependent protein S
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07225
  • Protein Name
    Vitamin K-dependent protein S
  • Scientific Description
    This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Protein S using anti-Protein S antibody (A01568-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein S antigen affinity purified polyclonal antibody (Catalog # A01568-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein S at approximately 75KD. The expected band size for Protein S is at 75KD.
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