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Western Blot analysis of Jurkat cells using PRP19 Polyclonal Antibody
Western Blot analysis of Jurkat cells using PRP19 Polyclonal Antibody
Western Blot analysis of Jurkat cells using PRP19 Polyclonal Antibody

PRPF19 Antibody

CSB-PA003855
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRPF19
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRPF19 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27339
  • Target name
    PRPF19
  • Target description
    pre-mRNA processing factor 19
  • Target synonyms
    hPSO4; NMP200; nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19; pre-mRNA-processing factor 19; PRP19; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog; PSO4; psoralen 4; RING-type E3 ubiquitin transferase PRP19; senescence evasion factor; SNEV; UBOX4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMS4
  • Protein Name
    Pre-mRNA-processing factor 19
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRPF19 using anti-PRPF19 antibody (A02434-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF19 antigen affinity purified polyclonal antibody (Catalog # A02434-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF19 at approximately 54 kDa. The expected band size for PRPF19 is at 55 kDa.
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