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PRPF19 / PRP19 Antibody

LS-C747685
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPRPF19
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRPF19 / PRP19 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 55kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), IP (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 55kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27339
  • Target name
    PRPF19
  • Target description
    pre-mRNA processing factor 19
  • Target synonyms
    NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4, pre-mRNA-processing factor 19, PRP19/PSO4 homolog, PRP19/PSO4 pre-mRNA processing factor 19 homolog, RING-type E3 ubiquitin transferase PRP19, nuclear matrix protein 200, nuclear matrix protein NMP200 related to splicing factor PRP19, psoralen 4, senescence evasion factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRPF19 using anti-PRPF19 antibody (A02434-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF19 antigen affinity purified polyclonal antibody (Catalog # A02434-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF19 at approximately 54 kDa. The expected band size for PRPF19 is at 55 kDa.
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