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WB analysis of various sample lysates using GTX66476 PRPF4 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66476 PRPF4 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66476 PRPF4 antibody. Dilution : 1:1000 Loading : 25microg per lane

PRPF4 antibody

GTX66476
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRPF4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRPF4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9128
  • Target name
    PRPF4
  • Target description
    pre-mRNA splicing tri-snRNP complex factor PRPF4
  • Target synonyms
    HPRP4, HPRP4P, PRP4, Prp4p, RP70, SNRNP60, U4/U6 small nuclear ribonucleoprotein Prp4, PRP4 homolog, PRP4 pre-mRNA processing factor 4 homolog, PRP4/STK/WD splicing factor, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4, pre-mRNA processing factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43172
  • Protein Name
    U4/U6 small nuclear ribonucleoprotein Prp4
  • Scientific Description
    The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF4 antigen affinity purified polyclonal antibody (Catalog # A08225-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF4 at approximately 55 kDa. The expected band size for PRPF4 is at 58kDa.
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Western blot All lanes: PRPF4 antibody at 2.87microg/ml Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
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