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Western Blot Positive WB detected in: Mouse heart tissue All lanes: PSAP antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Mouse heart tissue All lanes: PSAP antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Mouse heart tissue All lanes: PSAP antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa

PSAP Antibody

CSB-PA018836DA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPSAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSAP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5660
  • Target name
    PSAP
  • Target description
    prosaposin
  • Target synonyms
    GLBA, PARK24, PSAPD, SAP1, SAP2, prosaposin, precursor of saposins, proactivator polypeptide, saposin-A, saposin-B, saposin-C, saposin-D, sphingolipid activator protein-1, sphingolipid activator protein-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07602
  • Protein Name
    Prosaposin
  • Scientific Description
    The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSAP using anti-PSAP antibody (A00937-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAP antigen affinity purified polyclonal antibody (Catalog # A00937-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAP at approximately 70 kDa. The expected band size for PSAP is at 58 kDa.
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