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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: PSMA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 21 kDa Observed band size: 27 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: PSMA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 21 kDa Observed band size: 27 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: PSMA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 21 kDa Observed band size: 27 kDa

PSMA5 Antibody

CSB-PA018870LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPSMA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5686
  • Target name
    PSMA5
  • Target description
    proteasome 20S subunit alpha 5
  • Target synonyms
    epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; macropain zeta chain; multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; proteasome subunit alpha 5; proteasome subunit alpha type-5; proteasome subunit zeta; proteasome zeta chain; PSC5; ZETA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28066
  • Protein Name
    Proteasome subunit alpha type-5
  • Scientific Description
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMA5 using anti-PSMA5 antibody (A07499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA5 antigen affinity purified polyclonal antibody (Catalog # A07499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA5 at approximately 26 kDa. The expected band size for PSMA5 is at 26 kDa.
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Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Liver Tissue lysates; Lane 4: Rat Thymus tissue lysates probed with PSMA5 Polyclonal Antibody,Unconjugated (bs-9354R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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