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PSMB7 Antibody

LS-C749754
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPSMB7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PSMB7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 15kDa/29kDa, while the observed MW by Western blot was 30kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 15kDa/29kDa, while the observed MW by Western blot was 30kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5695
  • Target name
    PSMB7
  • Target description
    proteasome 20S subunit beta 7
  • Target synonyms
    epididymis secretory sperm binding protein; macropain chain Z; multicatalytic endopeptidase complex chain Z; proteasome (prosome, macropain) subunit, beta type, 7; proteasome catalytic subunit 2; proteasome subunit alpha; proteasome subunit beta 7; proteasome subunit beta type-7; proteasome subunit beta2; proteasome subunit Z; protein serine kinase c17; Z
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6; human HepG2 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 7/PSMB7 antigen affinity purified polyclonal antibody (Catalog # A08095-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 7/PSMB7 at approximately 26 kDa. The expected band size for Proteasome 20S Beta 7/PSMB7 is at 26 kDa.
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