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Western Blot analysis of 293 cells using PSMD2 Polyclonal Antibody
Western Blot analysis of 293 cells using PSMD2 Polyclonal Antibody
Western Blot analysis of 293 cells using PSMD2 Polyclonal Antibody

PSMD2 Antibody

CSB-PA003869
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMD2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5708
  • Target name
    PSMD2
  • Target description
    proteasome 26S subunit ubiquitin receptor, non-ATPase 2
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 2; 55.11 protein; P97; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; proteasome 26S subunit, non-ATPase 2; protein 55.11; RPN1; S2; TNFR-associated protein 2; TRAP2; tumor necrosis factor type 1 receptor-associated protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13200
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSMD2 using anti-PSMD2 antibody (A06642-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD2 antigen affinity purified polyclonal antibody (Catalog # A06642-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD2 at approximately 97 kDa. The expected band size for PSMD2 is at 97,100 kDa.
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