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WB analysis of human skin lysate using GTX89325 Psoriasin antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human skin lysate using GTX89325 Psoriasin antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human skin lysate using GTX89325 Psoriasin antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

Psoriasin antibody, Internal

GTX89325
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetS100A7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Psoriasin antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.3-1microg/ml. IHC-P: 3.75microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6278
  • Target name
    S100A7
  • Target description
    S100 calcium binding protein A7
  • Target synonyms
    protein S100-A7; PSOR1; psoriasin 1; S100A7c
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP31151
  • Protein Name
    Protein S100-A7
  • Scientific Description
    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities. [provided by RefSeq, Nov 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Psoriasin using anti-Psoriasin antibody (A02369-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat RH35 cell lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Psoriasin antigen affinity purified polyclonal antibody (Catalog # A02369-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Psoriasin at approximately 17KD. The expected band size for Psoriasin is at 11KD.
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