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ProductsBack/PTGES3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using P23 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using P23 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using P23 Polyclonal Antibody at dilution 1:50

PTGES3 Polyclonal Antibody

E-AB-14420
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PTGES3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    Prostaglandin E synthase 3 (cytosolic)is anenzymethat in humans is encoded by the PTGES3gene. The protein encoded by this gene is also known as p23 which functions as a chaperone which is required for proper functioning of the glucocorticoid and other steroid receptors. Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGES3 using anti-PTGES3 antibody (A04136-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human THP-1 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates, Lane 11: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES3 antigen affinity purified polyclonal antibody (Catalog # A04136-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGES3 at approximately 23KD. The expected band size for PTGES3 is at 23KD.
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