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PTGIS Antibody

CSB-PA040063
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPTGIS
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTGIS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5740
  • Target name
    PTGIS
  • Target description
    prostaglandin I2 synthase
  • Target synonyms
    CYP8, CYP8A1, PGIS, PTGI, prostacyclin synthase, cytochrome P450, family 8, subfamily A, polypeptide 1, hydroperoxy icosatetraenoate dehydratase, prostaglandin I2 (prostacyclin) synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16647
  • Protein Name
    Prostacyclin synthase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGIS/PGIS using anti-PTGIS/PGIS antibody (A05053-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGIS/PGIS antigen affinity purified polyclonal antibody (Catalog # A05053-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGIS/PGIS at approximately 57 kDa. The expected band size for PTGIS/PGIS is at 57 kDa.
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