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Western Blot analysis of K562 cells using PTGR2 Polyclonal Antibody
Western Blot analysis of K562 cells using PTGR2 Polyclonal Antibody
Western Blot analysis of K562 cells using PTGR2 Polyclonal Antibody

PTGR2 Antibody

CSB-PA007365
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTGR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTGR2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID145482
  • Target name
    PTGR2
  • Target description
    prostaglandin reductase 2
  • Target synonyms
    15-oxoprostaglandin 13-reductase; 15-oxoprostaglandin-delta13-reductase; epididymis secretory protein Li 298; epididymis secretory sperm binding protein; HEL-S-298; PGR2; PRG-2; prostaglandin reductase 2; ZADH1; zinc binding alcohol dehydrogenase, domain containing 1; zinc-binding alcohol dehydrogenase domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8N7
  • Protein Name
    Prostaglandin reductase 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGR2 using anti-PTGR2 antibody (A10106-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR2 antigen affinity purified polyclonal antibody (Catalog # A10106-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGR2 at approximately 40 kDa. The expected band size for PTGR2 is at 40 kDa.
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