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WB analysis of K562, HUVEC, and HT-29 cell lysates using GTX87478 ZADH1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562, HUVEC, and HT-29 cell lysates using GTX87478 ZADH1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562, HUVEC, and HT-29 cell lysates using GTX87478 ZADH1 antibody. The lane on the right is blocked with the synthesized peptide.

ZADH1 antibody

GTX87478
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPTGR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZADH1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID145482
  • Target name
    PTGR2
  • Target description
    prostaglandin reductase 2
  • Target synonyms
    HEL-S-298, PGR2, ZADH1, prostaglandin reductase 2, 15-oxoprostaglandin 13-reductase, 15-oxoprostaglandin-delta13-reductase, PRG-2, epididymis secretory protein Li 298, epididymis secretory sperm binding protein, zinc binding alcohol dehydrogenase, domain containing 1, zinc-binding alcohol dehydrogenase domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8N7
  • Protein Name
    Prostaglandin reductase 2
  • Scientific Description
    This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGR2 using anti-PTGR2 antibody (A10106-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR2 antigen affinity purified polyclonal antibody (Catalog # A10106-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGR2 at approximately 40 kDa. The expected band size for PTGR2 is at 40 kDa.
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