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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

BBC3/PUMA Polyclonal Antibody

BS-1573R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBBC3
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Overview

  • Supplier
    Bioss
  • Product Name
    BBC3/PUMA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID27113
  • Target name
    BBC3
  • Target description
    BCL2 binding component 3
  • Target synonyms
    JFY-1, JFY1, PUMA, bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, p53-upregulated modulator of apoptosis
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PG8
  • Protein Name
    Bcl-2-binding component 3, isoforms 3/4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Su C, Chen Y, et al. Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats. Mol Med Rep. 2019,20(5):4101-4110. doi: 10.3892/mmr.2019.10674
    Read this paper
  • Liu S, Shen M, Li C, et al. PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology. 2019,131:89-95. doi: 10.1016/j.theriogenology.2019.03.023
    Read this paper
  • Su LY, Shi YX, Yan MR, et al. Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway. Acta Pharmacol Sin. 2015,36(12):1514-9. doi: 10.1038/aps.2015.80
    Read this paper
  • Qiu S, Cai Y, Gao X, et al. A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53. Cancer Lett. 2015,356(2 Pt B):910-7. doi: 10.1016/j.canlet.2014.10.044
    Read this paper

Datasheet

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Figure 1. Western blot analysis of PUMA/BBC3 using anti-PUMA/BBC3 antibody (A04899-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUMA/BBC3 antigen affinity purified polyclonal antibody (Catalog # A04899-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUMA/BBC3 at approximately 18 kDa. The expected band size for PUMA/BBC3 is at 18 kDa.
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