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Purified Anti-Mouse CD19 Monoclonal Antibody

ORB1187207
Biorbyt
ApplicationsFlow Cytometry, ImmunoPrecipitation
Product group Antibodies
ReactivityMouse
TargetCd19
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Purified Anti-Mouse CD19 Monoclonal Antibody
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, ImmunoPrecipitation
  • Applications Supplier
    FC, IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D3
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12478
  • Target name
    Cd19
  • Target description
    CD19 antigen
  • Target synonyms
    B-lymphocyte antigen CD19, differentiation antigen CD19
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP25918
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    Rat monoclonal antibody to CD19
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human K562 whole cell lysates, Lane 6: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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