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Purified Anti-Mouse CD80 Antibody

RD20093F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd80
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    Purified Anti-Mouse CD80 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    16-10A1
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12519
  • Target name
    Cd80
  • Target description
    CD80 antigen
  • Target synonyms
    activation B7-1 antigen; B7 protein; B71; Cd28; Cd28l; Ly5; Ly-5; Ly53; Ly-53; MIC17; T-lymphocyte activation antigen CD80; TSA1
  • Host
    Hamster
  • Isotype
    IgG
  • Scientific Description
    CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd80 using anti-Cd80 antibody (A00196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd80 antigen affinity purified polyclonal antibody (Catalog # A00196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd80 at approximately 70 kDa. The expected band size for Cd80 is at 33 kDa.
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