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ProductsBack/PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4H5]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PUS7 plasmid (RC203902, hPUS7), mouse PUS7 plasmid (MR209845, mPUS7) using anti-PUS7 antibody TA501823 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PUS7 plasmid (RC203902, hPUS7), mouse PUS7 plasmid (MR209845, mPUS7) using anti-PUS7 antibody TA501823 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PUS7 plasmid (RC203902, hPUS7), mouse PUS7 plasmid (MR209845, mPUS7) using anti-PUS7 antibody TA501823 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4H5]

CF501823
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetPUS7
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4H5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4H5
  • Gene ID54517
  • Target name
    PUS7
  • Target description
    pseudouridine synthase 7
  • Target synonyms
    IDDABS, pseudouridylate synthase 7 homolog, pseudouridylate synthase 7 (putative)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96PZ0
  • Protein Name
    Pseudouridylate synthase 7 homolog
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PUS7 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PUS7 using anti-PUS7 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUS7 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUS7 at approximately 75 kDa. The expected band size for PUS7 is at 75 kDa.
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