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ProductsBack/PUS7 Mouse Monoclonal Antibody [Clone ID: OTI5F12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7. Positive lysates LY412749 (100ug) and LC412749 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7. Positive lysates LY412749 (100ug) and LC412749 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7. Positive lysates LY412749 (100ug) and LC412749 (20ug) can be purchased separately from OriGene.

PUS7 Mouse Monoclonal Antibody [Clone ID: OTI5F12]

CF501815
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetPUS7
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PUS7 Mouse Monoclonal Antibody [Clone ID: OTI5F12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5F12
  • Gene ID54517
  • Target name
    PUS7
  • Target description
    pseudouridine synthase 7
  • Target synonyms
    IDDABS, pseudouridylate synthase 7 homolog, pseudouridylate synthase 7 (putative)
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ96PZ0
  • Protein Name
    Pseudouridylate synthase 7 homolog
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PUS7 mouse monoclonal antibody, clone OTI5F12 (formerly 5F12)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PUS7 using anti-PUS7 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUS7 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUS7 at approximately 75 kDa. The expected band size for PUS7 is at 75 kDa.
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