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Western Blot analysis of KB cells using CD155 Polyclonal Antibody
Western Blot analysis of KB cells using CD155 Polyclonal Antibody
Western Blot analysis of KB cells using CD155 Polyclonal Antibody

PVR Antibody

CSB-PA006288
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPVR
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Overview

  • Supplier
    Cusabio
  • Product Name
    PVR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5817
  • Target name
    PVR
  • Target description
    PVR cell adhesion molecule
  • Target synonyms
    CD155; HVED; NECL5; Necl-5; nectin-like protein 5; poliovirus receptor; PVS; TAGE4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15151
  • Protein Name
    Poliovirus receptor
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (A00664-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HT1080 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Poliovirus Receptor/PVR antigen affinity purified polyclonal antibody (Catalog # A00664-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80KD. The expected band size for Poliovirus Receptor/PVR is at 70-80KD.
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