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WB analysis of various sample lysates using GTX33447 PYCR1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33447 PYCR1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33447 PYCR1 antibody. Dilution : 1:1000 Loading : 25microg per lane

PYCR1 antibody

GTX33447
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPYCR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PYCR1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5831
  • Target name
    PYCR1
  • Target description
    pyrroline-5-carboxylate reductase 1
  • Target synonyms
    ARCL2B, ARCL3B, P5C, P5CR, PIG45, PP222, PRO3, PYCR, pyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, proliferation-inducing protein 45
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32322
  • Protein Name
    Pyrroline-5-carboxylate reductase 1, mitochondrial
  • Scientific Description
    This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR1 antigen affinity purified polyclonal antibody (Catalog # A06018-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR1 at approximately 35 kDa. The expected band size for PYCR1 is at 33,35 kDa.
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