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Western blot analysis of extracts of various cell lines using PYCR1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PYCR1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PYCR1 Polyclonal Antibody at 1:1000 dilution.

PYCR1 Polyclonal Antibody

E-AB-64571
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PYCR1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR1 antigen affinity purified polyclonal antibody (Catalog # A06018-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR1 at approximately 35 kDa. The expected band size for PYCR1 is at 33,35 kDa.
Product group Antibodies
Boster Bio
Anti-PYCR1 Antibody Picoband(r)A06018-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPYCR1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PYCR1 Antibody144-06959
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPYCR1
  • SizePrice
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019115HA01HU at dilution of 1:100
Product group Antibodies
Cusabio
PYCR1 AntibodyCSB-PA019115HA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPYCR1
  • SizePrice
Product group Antibodies
Assay Genie
PYCR1 AntibodyCAB6959
ReactivityHuman
TargetPYCR1
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Product group Antibodies
Atlas Antibodies
Anti-PYCR1 AntibodyHPA047660
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPYCR1
  • SizePrice
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PYCR1 antibody (GTX114693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
PYCR1 antibodyGTX114693
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPYCR1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PYCR1 AntibodyLS-C748400
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPYCR1
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-PYCR1 AntibodyA31777
ApplicationsWestern Blot, ImmunoHistoChemistry
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