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ProductsBack/PYCR2 Mouse Monoclonal Antibody [Clone ID: OTI3D1]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and PYCR2-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-PYCR2 monoclonal antibody TA501901(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and PYCR2-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-PYCR2 monoclonal antibody TA501901(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and PYCR2-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-PYCR2 monoclonal antibody TA501901(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

PYCR2 Mouse Monoclonal Antibody [Clone ID: OTI3D1]

CF501901
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPYCR2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PYCR2 Mouse Monoclonal Antibody [Clone ID: OTI3D1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3D1
  • Gene ID29920
  • Target name
    PYCR2
  • Target description
    pyrroline-5-carboxylate reductase 2
  • Target synonyms
    HLD10, P5CR2, pyrroline-5-carboxylate reductase 2, P5C reductase 2, pyrroline-5-carboxylate reductase family member 2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ96C36
  • Protein Name
    Pyrroline-5-carboxylate reductase 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PYCR2 mouse monoclonal antibody, clone OTI3D1 (formerly 3D1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYCR2 using anti-PYCR2 antibody (A10327-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR2 antigen affinity purified polyclonal antibody (Catalog # A10327-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR2 at approximately 35 kDa. The expected band size for PYCR2 is at 34,35 kDa.
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