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ProductsBack/PYCR3 Mouse Monoclonal Antibody [Clone ID: OTI1B12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL. Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL. Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL. Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.

PYCR3 Mouse Monoclonal Antibody [Clone ID: OTI1B12]

CF502078
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetPYCR3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PYCR3 Mouse Monoclonal Antibody [Clone ID: OTI1B12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B12
  • Gene ID65263
  • Target name
    PYCR3
  • Target description
    pyrroline-5-carboxylate reductase 3
  • Target synonyms
    PYCRL, pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, pyrroline-5-carboxylate reductase-like
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ53H96
  • Protein Name
    Pyrroline-5-carboxylate reductase 3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PYCRL mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYCRL/PYCR3 using anti-PYCRL/PYCR3 antibody (A32428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCRL/PYCR3 antigen affinity purified polyclonal antibody (A32428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCRL/PYCR3 at approximately 29 kDa. The expected band size for PYCRL/PYCR3 is at 29 kDa.
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WB analysis of HEK293T cells transfected with PYCRL plasmid (Right) or empty vector (Left) for 48 hrs using GTX83750 PYCRL antibody [2E10]. Loading : 5 ug per lane
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