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Pygopus 2 Recombinant Antibody

BSM-61113R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPYGO2
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Overview

  • Supplier
    Bioss
  • Product Name
    Pygopus 2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), FCM(1ug/Test), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID90780
  • Target name
    PYGO2
  • Target description
    pygopus family PHD finger 2
  • Target synonyms
    1190004M21Rik, pygopus homolog 2, pygopus 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRQ0
  • Protein Name
    Pygopus homolog 2
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Pygopus 2 using anti-Pygopus 2 antibody (M00283). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: huamn U2OS whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pygopus 2 antigen affinity purified monoclonal antibody (M00283) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pygopus 2 at approximately 50 kDa. The expected band size for Pygopus 2 is at 41 kDa.
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