Western blot using GeneTexs affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 microg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4o C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Western blot using GeneTexs affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 microg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4o C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Pyruvate dehydrogenase kinase 1 / PDK1 antibody
GTX22495
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDK1
Overview
- SupplierGeneTex
- Product NamePyruvate dehydrogenase kinase 1 / PDK1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:100000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.3 mg/ml
- ConjugateUnconjugated
- Gene ID5163
- Target namePDK1
- Target descriptionpyruvate dehydrogenase kinase 1
- Target synonyms[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme 1
- HostRabbit
- IsotypeIgG
- Protein IDQ15118
- Protein Name[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
- Scientific DescriptionPDK 1 (3-Phosphoinositide-Dependant Protein Kinase 1) phosphorylates AGC kinases. PDK 1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK 1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203