
WB analysis of rat heart lysate using GTX89378 Pyruvate dehydrogenase kinase 1 / PDK1 antibody, Internal. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
Pyruvate dehydrogenase kinase 1 / PDK1 antibody, Internal
GTX89378
ApplicationsWestern Blot
Product group Antibodies
TargetPDK1
Overview
- SupplierGeneTex
- Product NamePyruvate dehydrogenase kinase 1 / PDK1 antibody, Internal
- Delivery Days Customer7
- Application Supplier NoteWB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.50 mg/ml
- ConjugateUnconjugated
- Gene ID5163
- Target namePDK1
- Target descriptionpyruvate dehydrogenase kinase 1
- Target synonyms[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial, PDH kinase 1, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1
- HostGoat
- IsotypeIgG
- Protein IDQ15118
- Protein Name[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
- Scientific DescriptionPyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![Western blot using GeneTexs affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 microg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4o C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX22495/GTX22495_20160330_WB_w_23060620_134.webp)
![FACS analysis of Lovo cells using GTX60386 PDK1 antibody [4A11]. Green : PDK1 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60386/GTX60386_20170912_FACS_w_23061123_454.webp)


