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ProductsBack/Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1F3]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PKLR plasmid (RC206455, hPKLR), mouse PKLR plasmid (MR215886, mPKLR) using anti-PKLR antibody TA501412 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PKLR plasmid (RC206455, hPKLR), mouse PKLR plasmid (MR215886, mPKLR) using anti-PKLR antibody TA501412 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PKLR plasmid (RC206455, hPKLR), mouse PKLR plasmid (MR215886, mPKLR) using anti-PKLR antibody TA501412 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CF501412
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPKLR
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1F3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1F3
  • Gene ID5313
  • Target name
    PKLR
  • Target description
    pyruvate kinase L/R
  • Target synonyms
    CNSHA2, PK1, PKL, PKRL, RPK, pyruvate kinase PKLR, R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase isozyme R/L, pyruvate kinase isozymes L/R, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and RBC, pyruvate kinase, liver and blood cell, red cell/liver pyruvate kinase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP30613
  • Protein Name
    Pyruvate kinase PKLR
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PKLR mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PKLR using anti-PKLR antibody (PB9499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody (Catalog # PB9499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKLR at approximately 62 kDa. The expected band size for PKLR is at 62 kDa.
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