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ProductsBack/Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1H2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Positive lysates LY400113 (100ug) and LC400113 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Positive lysates LY400113 (100ug) and LC400113 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Positive lysates LY400113 (100ug) and LC400113 (20ug) can be purchased separately from OriGene.

Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1H2]

CF501416
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPKLR
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1H2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1H2
  • Gene ID5313
  • Target name
    PKLR
  • Target description
    pyruvate kinase L/R
  • Target synonyms
    CNSHA2, PK1, PKL, PKRL, RPK, pyruvate kinase PKLR, R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase isozyme R/L, pyruvate kinase isozymes L/R, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and RBC, pyruvate kinase, liver and blood cell, red cell/liver pyruvate kinase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP30613
  • Protein Name
    Pyruvate kinase PKLR
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PKLR mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PKLR using anti-PKLR antibody (PB9499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody (Catalog # PB9499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKLR at approximately 62 kDa. The expected band size for PKLR is at 62 kDa.
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