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ProductsBack/QPRT antibody [AT24C4]
ICC/IF analysis of Hep3B cells using GTX57665 QPRT antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
ICC/IF analysis of Hep3B cells using GTX57665 QPRT antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
ICC/IF analysis of Hep3B cells using GTX57665 QPRT antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100

QPRT antibody [AT24C4]

GTX57665
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetQPRT
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Overview

  • Supplier
    GeneTex
  • Product Name
    QPRT antibody [AT24C4]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT24C4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23475
  • Target name
    QPRT
  • Target description
    quinolinate phosphoribosyltransferase
  • Target synonyms
    epididymis secretory sperm binding protein Li 90n; HEL-S-90n; nicotinate-nucleotide pyrophosphorylase (carboxylating); nicotinate-nucleotide pyrophosphorylase [carboxylating]; QPRTase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ15274
  • Protein Name
    Nicotinate-nucleotide pyrophosphorylase [carboxylating]
  • Scientific Description
    This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimers disease, and Huntingtons disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of QPRT using anti-QPRT antibody (A09896). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QPRT antigen affinity purified polyclonal antibody (Catalog # A09896) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for QPRT at approximately 31-35 kDa. The expected band size for QPRT is at 31 kDa.
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FACS analysis of HEK293T cells transfected with either QPRT plasmid(Red) or empty vector control plasmid(Blue) using GTX83744 QPRT antibody [4B4].
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IHC image of CSB-PA621868LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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QPRT AntibodyCSB-PA621868LA01HU
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