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ProductsBack/Quiescin Q6 (QSOX1) Rabbit Polyclonal Antibody
Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-3: 293T Hela and 231 cell lysates Primary antibody: TA364948 (QSOX1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-3: 293T Hela and 231 cell lysates Primary antibody: TA364948 (QSOX1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-3: 293T Hela and 231 cell lysates Primary antibody: TA364948 (QSOX1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds

Quiescin Q6 (QSOX1) Rabbit Polyclonal Antibody

TA364948
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetQSOX1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Quiescin Q6 (QSOX1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5768
  • Target name
    QSOX1
  • Target description
    quiescin sulfhydryl oxidase 1
  • Target synonyms
    Q6; QSCN6; quiescin Q6 sulfhydryl oxidase 1; sulfhydryl oxidase 1; testis tissue sperm-binding protein Li 62n; thiol oxidase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00391
  • Protein Name
    Sulfhydryl oxidase 1
  • Scientific Description
    QSOX1 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Quiescin Q6/QSOX1 using anti-Quiescin Q6/QSOX1 antibody (A04549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Quiescin Q6/QSOX1 antigen affinity purified polyclonal antibody (Catalog # A04549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Quiescin Q6/QSOX1 at approximately 105 kDa. The expected band size for Quiescin Q6/QSOX1 is at 83 kDa.
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