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ProductsBack/RAB11A (6C8) Monoclonal Antibody
Lane 1: HeLa; Lane 2: Jurkat; Lane 3: C2C12; Lane 4: PC-12; Lane 5: rat brain; Lane 6: 293T/17 lysates (20µg) probed with bsm-51322M RAB11A (6C8) Monoclonal Antibody at 1:4000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HeLa; Lane 2: Jurkat; Lane 3: C2C12; Lane 4: PC-12; Lane 5: rat brain; Lane 6: 293T/17 lysates (20µg) probed with bsm-51322M RAB11A (6C8) Monoclonal Antibody at 1:4000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HeLa; Lane 2: Jurkat; Lane 3: C2C12; Lane 4: PC-12; Lane 5: rat brain; Lane 6: 293T/17 lysates (20µg) probed with bsm-51322M RAB11A (6C8) Monoclonal Antibody at 1:4000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

RAB11A (6C8) Monoclonal Antibody

BSM-51322M
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRAB11A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    RAB11A (6C8) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6C8
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID8766
  • Target name
    RAB11A
  • Target description
    RAB11A, member RAS oncogene family
  • Target synonyms
    RAB 11A, member oncogene family; rab-11; ras-related protein Rab-11A; YL8
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP62491
  • Protein Name
    Ras-related protein Rab-11A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rab11A using anti-Rab11A antibody (PB9789). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human MDA-MB-453 whole cell lysates, Lane 8: human Jurkat whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: rat brain tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (Catalog # PB9789) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.
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