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RAB11A Antibody

LS-C830357
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAB11A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RAB11A Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:25 - 1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8766
  • Target name
    RAB11A
  • Target description
    RAB11A, member RAS oncogene family
  • Target synonyms
    YL8, ras-related protein Rab-11A, RAB 11A, member oncogene family, rab-11
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rab11A using anti-Rab11A antibody (PB9789). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human MDA-MB-453 whole cell lysates, Lane 8: human Jurkat whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: rat brain tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (Catalog # PB9789) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.
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