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WB analysis of various sample lysates using GTX64901 RAB18 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64901 RAB18 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64901 RAB18 antibody. Dilution : 1:1000 Loading : 25microg per lane

RAB18 antibody

GTX64901
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRAB18
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Overview

  • Supplier
    GeneTex
  • Product Name
    RAB18 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22931
  • Target name
    RAB18
  • Target description
    RAB18, member RAS oncogene family
  • Target synonyms
    RAB18LI1, WARBM3, ras-related protein Rab-18, RAB18 small GTPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP72
  • Protein Name
    Ras-related protein Rab-18
  • Scientific Description
    The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies is zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rab18 using anti-Rab18 antibody (PB9791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: 293T Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab18 antigen affinity purified polyclonal antibody (Catalog # PB9791) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab18 at approximately 23 kDa. The expected band size for Rab18 is at 23 kDa.
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