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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA019178ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA019178ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA019178ESR1HU at dilution of 1:100

RAB27B Antibody

CSB-PA019178ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAB27B
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB27B Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5874
  • Target name
    RAB27B
  • Target description
    RAB27B, member RAS oncogene family
  • Target synonyms
    C25KG, ras-related protein Rab-27B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00194
  • Protein Name
    Ras-related protein Rab-27B
  • Scientific Description
    May be involved in targeting uroplakins to urothelial apical membranes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAB27B using anti-RAB27B antibody (A04890-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27B antigen affinity purified polyclonal antibody (Catalog # A04890-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB27B at approximately 25 kDa. The expected band size for RAB27B is at 25 kDa.
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Lane 1: DU145 Cell lysates; Lane 2: mouse stomach Cell lysates; probed with RAB27B (4G7) Monoclonal Antibody, unconjugated (bsm-51331M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Immunohistochemistry analysis in human prostate and tonsil tissues using HPA019849 antibody. Corresponding RAB27B RNA-seq data are presented for the same tissues.
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