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WB analysis of various sample lysates using GTX64615 RAB27B antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64615 RAB27B antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64615 RAB27B antibody. Dilution : 1:1000 Loading : 25microg per lane

RAB27B antibody

GTX64615
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRAB27B
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Overview

  • Supplier
    GeneTex
  • Product Name
    RAB27B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5874
  • Target name
    RAB27B
  • Target description
    RAB27B, member RAS oncogene family
  • Target synonyms
    C25KG, ras-related protein Rab-27B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00194
  • Protein Name
    Ras-related protein Rab-27B
  • Scientific Description
    Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Li R, Dong C, Jiang K, et al. Rab27B enhances drug resistance in hepatocellular carcinoma by promoting exosome-mediated drug efflux. Carcinogenesis. 2020,41(11):1583-1591. doi: 10.1093/carcin/bgaa029
    Read this paper
  • Sun R, Dong C, Li R, et al. Proteomic Analysis Reveals that EPHX1 Contributes to 5-Fluorouracil Resistance in a Human Hepatocellular Carcinoma Cell Line. Proteomics Clin Appl. 2020,14(4):e1900080. doi: 10.1002/prca.201900080
    Read this paper

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Figure 1. Western blot analysis of RAB27B using anti-RAB27B antibody (A04890-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27B antigen affinity purified polyclonal antibody (Catalog # A04890-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB27B at approximately 25 kDa. The expected band size for RAB27B is at 25 kDa.
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