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Western Blot analysis of 293 cells using Rab 34 Polyclonal Antibody
Western Blot analysis of 293 cells using Rab 34 Polyclonal Antibody
Western Blot analysis of 293 cells using Rab 34 Polyclonal Antibody

RAB34 Antibody

CSB-PA003895
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAB34
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB34 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83871
  • Target name
    RAB34
  • Target description
    RAB34, member RAS oncogene family
  • Target synonyms
    NARR; nine amino-acid residue-repeats; RAB39; RAH; ras-related protein Rab-34; ras-related protein Rab-39; ras-related protein Rah
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZG1
  • Protein Name
    Ras-related protein Rab-34
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB34 using anti-RAB34 antibody (A11097-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB34 antigen affinity purified polyclonal antibody (Catalog # A11097-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB34 at approximately 29 kDa. The expected band size for RAB34 is at 29 kDa.
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