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Western Blot analysis of K562 cells using RAB3IP Polyclonal Antibody
Western Blot analysis of K562 cells using RAB3IP Polyclonal Antibody
Western Blot analysis of K562 cells using RAB3IP Polyclonal Antibody

RAB3IP Antibody

CSB-PA006887
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAB3IP
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB3IP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID117177
  • Target name
    RAB3IP
  • Target description
    RAB3A interacting protein
  • Target synonyms
    RABIN3, RABIN8, rab-3A-interacting protein, SSX2 interacting protein, rab3A-interacting protein, rabin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96QF0
  • Protein Name
    Rab-3A-interacting protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAB3IP using anti-RAB3IP antibody (A09085-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3IP antigen affinity purified polyclonal antibody (Catalog # A09085-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB3IP at approximately 55 kDa. The expected band size for RAB3IP is at 53 kDa.
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