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WB analysis of various sample lysates using GTX32828 RAB5C antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32828 RAB5C antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32828 RAB5C antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

RAB5C antibody

GTX32828
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRAB5C
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Overview

  • Supplier
    GeneTex
  • Product Name
    RAB5C antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5878
  • Target name
    RAB5C
  • Target description
    RAB5C, member RAS oncogene family
  • Target synonyms
    L1880; RAB5C, member of RAS oncogene family; RAB5CL; RAB5L; RABL; ras-related protein Rab-5C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51148
  • Protein Name
    Ras-related protein Rab-5C
  • Scientific Description
    Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).[supplied by OMIM, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: A431 Cell lysates; Lane 2: HL-60 Cell lysates; Lane 3: U-2OS Cell lysates; probed with RAB5C (6C1) Monoclonal Antibody, unconjugated (bsm-51343M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of RAB5C/RABL using anti-RAB5C/RABL antibody (M05148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5C/RABL antigen affinity purified monoclonal antibody (Catalog # M05148-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5C/RABL at approximately 23 kDa. The expected band size for RAB5C/RABL is at 23 kDa.
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