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ProductsBack/Rabbit anti-4-1BBL/CD137L/TNFSF9 Recombinant Monoclonal Antibody [BLR148J]

Rabbit anti-4-1BBL/CD137L/TNFSF9 Recombinant Monoclonal Antibody [BLR148J]

A700-148
Bethyl Laboratories
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetTNFSF9
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-4-1BBL/CD137L/TNFSF9 Recombinant Monoclonal Antibody [BLR148J]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR148J
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8744
  • Target name
    TNFSF9
  • Target description
    TNF superfamily member 9
  • Target synonyms
    4-1BB-L, CD137L, TNLG5A, tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, CD137 ligand, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand 5A, tumor necrosis factor superfamily member 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41273
  • Protein Name
    Tumor necrosis factor ligand superfamily member 9
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 4-1BBL/Tnfsf9 using anti-4-1BBL/Tnfsf9 antibody (A06032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-4-1BBL/Tnfsf9 antigen affinity purified polyclonal antibody (Catalog # A06032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 4-1BBL/Tnfsf9 at approximately 27 kDa. The expected band size for 4-1BBL/Tnfsf9 is at 27 kDa.
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