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ProductsBack/Rabbit anti-AHSG/Fetuin-A Antibody, Affinity Purified

Rabbit anti-AHSG/Fetuin-A Antibody, Affinity Purified

A305-236A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetAHSG
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-AHSG/Fetuin-A Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID197
  • Target name
    AHSG
  • Target description
    alpha 2-HS glycoprotein
  • Target synonyms
    A2HS, AHS, APMR1, FETUA, HSGA, alpha-2-HS-glycoprotein, alpha-2-Z-globulin, ba-alpha-2-glycoprotein, fetuin-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02765
  • Protein Name
    Alpha-2-HS-glycoprotein
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AHSG using anti-AHSG antibody (PB9568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat plasma lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHSG antigen affinity purified polyclonal antibody (Catalog # PB9568) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHSG at approximately 55-60 kDa. The expected band size for AHSG is at 39 kDa.
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