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Rabbit anti-Asunder Antibody, Affinity Purified

A303-575A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetINTS13
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Asunder Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55726
  • Target name
    INTS13
  • Target description
    integrator complex subunit 13
  • Target synonyms
    ASUN, C12orf11, GCT1, Mat89Bb, NET48, SPATA30, integrator complex subunit 13, asunder, spermatogenesis regulator homolog (Drosphila), cell cycle regulator Mat89Bb homolog, germ cell tumor 1, protein asunder homolog, sarcoma antigen NY-SAR-95, spermatogenesis associated 30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVM9
  • Protein Name
    Integrator complex subunit 13
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate All lanes: INTS13 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 69 kDa Observed band size: 81 kDa
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Figure 1. Western blot analysis of INTS13 using anti-INTS13 antibody (A32419-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS13 antigen affinity purified polyclonal antibody (Catalog # A32419-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTS13 at approximately 85 kDa. The expected band size for INTS13 is at 80 kDa.
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