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ProductsBack/Rabbit anti-Beta-2-adaptin Antibody, Affinity Purified

Rabbit anti-Beta-2-adaptin Antibody, Affinity Purified

A304-718A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetAP2B1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Beta-2-adaptin Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID163
  • Target name
    AP2B1
  • Target description
    adaptor related protein complex 2 subunit beta 1
  • Target synonyms
    ADTB2, AP105B, AP2-BETA, CLAPB1, AP-2 complex subunit beta, adapter-related protein complex 2 beta subunit, adapter-related protein complex 2 subunit beta, adaptin, beta 2 (beta), adaptor protein complex AP-2 subunit beta, adaptor related protein complex 2 beta 1 subunit, adaptor-related protein complex 2 subunit beta, beta-2-adaptin, beta-adaptin, beta2-adaptin, clathrin assembly protein complex 2 beta large chain, clathrin-associated/assembly/adaptor protein, large, beta 1, plasma membrane adaptor HA2/AP2 adaptin beta subunit, testicular tissue protein Li 22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63010
  • Protein Name
    AP-2 complex subunit beta
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AP2B1 using anti-AP2B1 antibody (A06544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2B1 antigen affinity purified polyclonal antibody (Catalog # A06544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2B1 at approximately 105 kDa. The expected band size for AP2B1 is at 105 kDa.
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